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Comparison of sequence data for Broadhaven (BRD) virus, a tick-borne orbivirus, and bluetongue virus (BTV), the type species of the genus, indicated that RNA segments 2 and 7 of BRD virus encode the two structural core proteins, VP2 and VP7, respectively. Segment 2 is 2792 nucleotides in length with a coding capacity for a protein (VP2) of 908 amino acids and a net charge of +8.5 at neutral pH. Segment 7 is 1174 nucleotides in length with a coding capacity for a protein (VP7) of 356 amino acids and a net charge of +11.5 at neutral pH. Comparison of the two sequences with BTV serotype 10 revealed amino acid identity of 35% between the product of segment 2 and BTV VP3, and 21% between the product of segment 7 and BTV VP7. The core proteins therefore show evidence of significant evolutionary divergence compared with that shown between different insect-borne orbiviruses. In particular, the amino terminus of BRD virus VP7 differed markedly from the equivalent region in VP7 of BTV and African horse sickness virus. This region is thought to interact with the outer capsid layer of insect-borne orbiviruses.

Original publication

DOI

10.1099/0022-1317-73-10-2585

Type

Journal article

Journal

J Gen Virol

Publication Date

10/1992

Volume

73 ( Pt 10)

Pages

2585 - 2590

Keywords

Amino Acid Sequence, Animals, Antigens, Viral, Base Sequence, Capsid, Capsid Proteins, Cells, Cultured, Ceratopogonidae, Genes, Viral, Insect Vectors, Molecular Sequence Data, Orbivirus, Sequence Homology, Amino Acid, Ticks, Viral Core Proteins, Viral Structural Proteins