Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Three orbiviruses of the Kemerovo serogroup, isolated from ticks from two sites on the Isle of May in Scotland, were plaque purified and the proteins and RNA induced in Vero cells compared. Two viruses (Mill Door/79 and Mill Door/81) from the same site differed in the migration of at least 7 segments of dsRNA. The third virus (North Clett/81) was clearly distinguished from Mill Door/79 and Mill Door/81 viruses in the migration of 6 dsRNA segments. Analysis of virus-induced polypeptides demonstrated minor molecular weight differences, but partial proteolysis failed to show significant variations in the proteins. Precipitation of radiolabelled virus-induced proteins by hyperimmune ascitic fluids did not distinguish between the isolates.

Type

Journal article

Journal

Acta Virol

Publication Date

01/1986

Volume

30

Pages

19 - 24

Keywords

Animals, Birds, Peptides, RNA, Viral, Reoviridae, Scotland, Serotyping, Species Specificity, Ticks, Viral Plaque Assay, Viral Proteins