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The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.

Type

Journal article

Journal

J Virol

Publication Date

02/1985

Volume

53

Pages

705 - 707

Keywords

Animals, Birds, Cell Line, Cercopithecus aethiops, Genes, Viral, RNA, Double-Stranded, RNA, Viral, Reoviridae, Scotland, Ticks, Viral Proteins, Virus Replication