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Three methods of isolating viruses from 10 tick pools were compared; none of the methods produced all 13 of the viruses isolated (7 viruses of the bunyaviridae and 6 orbiviruses). Inoculation of homogenised ticks into various cell lines was the most successful, yielding 11 virus isolations. Only 2 tick homogenates induced overt signs of infection following intra-cerebral inoculation of 2-day-old mice. However, when inoculated mouse brain was passaged in various cell lines, 8 of 12 isolations were made. The rates of success of the 3 methods of virus isolation appeared to vary according to the type and titre of the virus in the tick pool.

Type

Journal article

Journal

J Virol Methods

Publication Date

08/1984

Volume

9

Pages

27 - 33

Keywords

Animals, Birds, Brain, Bunyaviridae, Cell Line, Cells, Cultured, Cercopithecus aethiops, Chick Embryo, Cricetinae, Female, Kidney, Male, Mice, Microscopy, Electron, Reoviridae, Ticks, Virus Cultivation, Virus Replication, Xenopus laevis