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Apoptosis is an important mechanism to eliminate potentially tumorigenic cells. The tumor suppressor p53 plays a pivotal role in this process. Many tumors harbor mutant p53, but others evade its tumor-suppressive effects by altering the expression of proteins that regulate the p53 pathway. ASPP1 (apoptosis-stimulating protein of p53-1) is a key mediator of the nuclear p53 apoptotic response. Under basal conditions, ASPP1 is cytoplasmic. We report that, in response to oncogenic stress, the tumor suppressor Lats2 (large tumor suppressor 2) phosphorylates ASPP1 and drives its translocation into the nucleus. Together, Lats2 and ASPP1 shunt p53 to proapoptotic promoters and promote the death of polyploid cells. These effects are overridden by the Yap1 (Yes-associated protein 1) oncoprotein, which disrupts Lats2-ASPP1 binding and antagonizes the tumor-suppressing function of the Lats2/ASPP1/p53 axis.

Original publication

DOI

10.1101/gad.1954410

Type

Journal article

Journal

Genes Dev

Publication Date

01/11/2010

Volume

24

Pages

2420 - 2429

Keywords

Adaptor Proteins, Signal Transducing, Apoptosis, Apoptosis Regulatory Proteins, Blotting, Western, Cell Line, Cell Nucleus, Chromatin Immunoprecipitation, Cytoplasm, HCT116 Cells, Humans, Mutation, Phosphoproteins, Phosphorylation, Protein Binding, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference, Tumor Suppressor Protein p53, Tumor Suppressor Proteins