A rapid screening method for lipid-accumulating yeast using a replica-printing technique
Evans CT., Ratledge C., Gilbert SC.
A replica-printing technique has been developed which enables the tentative identification of a high lipid-containing yeast colony on an agar plate. The method involves a simple permeabilization and staining procedure, followed by destaining, and is able to detect colonies that accumulate lipid (that is those from Candida curvata, Lipomyces starkeyi and Trichosporon cutaneum) whilst easily distinguishing the latter from a low-lipid containing colony (Saccharomyces cerevisiae). The technique could not be successfully applied to the red yeast, Rhodosporidium toruloides. The method is simple and rapid and so provides for efficient screening and identification of high lipid-producing hybrids (protoplast fusion, recombinant DNA), mutants and natural isolates. © 1985.