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Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

Original publication

DOI

10.1038/s41556-018-0251-8

Type

Journal article

Journal

Nat Cell Biol

Publication Date

01/2019

Volume

21

Pages

72 - 84

Keywords

Animals, Cell Biology, Humans, Imaging, Three-Dimensional, Microscopy, Fluorescence, Molecular Biology, Reproducibility of Results