Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Interleukin 2 (IL-2)-deficient (IL-2-/-) mice develop hemolytic anemia and chronic inflammatory bowel disease. Importantly, the induction of disease in IL-2-deficient mice is critically dependent on CD4+ T cells. We have studied the requirements of T cells from IL-2-deficient mice for costimulation with B7 antigens. Stable B7-1 or B7-2 chinese hamster ovary (CHO) cell transfectants could synergize with anti-CD3 monoclonal antibody (mAb) to induce the proliferation of CD4+ T cells from IL-2-/- mutant mice. Further mechanistic studies established that B7-induced activation resulted in surface expression of the alpha chain of the IL-2 receptor. B7-induced proliferation occurred independently of IL-4 and was largely independent of the common gamma chain of the IL-2, IL-4, IL-7, IL-9, and IL-15 receptors. Finally, anti-B7-2 but not anti-B7-1 mAb was able to inhibit the activation of IL-2-/- T cells induced by anti-CD3 mAb in the presence of syngeneic antigen-presenting cells. The results of our experiments indicate that IL-2-/- CD4+ T cells remain responsive to B7 stimulation and raise the possibility that B7 antagonists have a role in the prevention/treatment of inflammatory bowel disease.

Original publication

DOI

10.1073/pnas.93.7.2903

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

02/04/1996

Volume

93

Pages

2903 - 2908

Keywords

Anemia, Hemolytic, Animals, Antibodies, Monoclonal, B7-1 Antigen, Base Sequence, CD3 Complex, CD4-Positive T-Lymphocytes, CHO Cells, Cricetinae, DNA Primers, Inflammatory Bowel Diseases, Interleukin-2, Kinetics, Lymph Nodes, Lymphocyte Activation, Mice, Mice, Mutant Strains, Molecular Sequence Data, Polymerase Chain Reaction, Spleen, Transfection